Skip to main content
. Author manuscript; available in PMC: 2016 Aug 21.
Published in final edited form as: ACS Chem Biol. 2015 May 22;10(8):1887–1896. doi: 10.1021/acschembio.5b00101

Figure 1.

Figure 1

Effect of HPI and other protease inhibitors on the ability of NS3 to unwind DNA. (A) Direct acting antivirals used in this study. (B) Ability of HPI to inhibit molecular beacon-based helicase assays (MBHAs). In MBHAs, NS3 separates a molecular beacon from a complementary strand so that fluorescence decreases with time. (C) Activity remaining, calculated from normalized initial velocities, in the presence of various NS3 protease inhibitors (data for boceprevir and danoprevir are omitted for clarity. Neither inhibit the reaction). Data were fitted to a normalized concentration-response equation using Graphpad Prism v.6. The IC50 value for HPI was 6±2 μM.