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. Author manuscript; available in PMC: 2016 Aug 17.
Published in final edited form as: Curr Biol. 2015 Jul 30;25(16):2177–2183. doi: 10.1016/j.cub.2015.06.063

Figure 1. Zeta-tubulin is a conserved tubulin that associates with TRiC/CCT.

Figure 1

A) Presence or absence of ZED module tubulins in representative organisms. B) Unrooted phylogenetic tree, based on Clustal Omega alignment. C–E) Sucrose gradient sedimentation of zeta-tubulin from C) A6 cells, D) eggs, and E) whole oviduct. Lysates were separated on 10–40% sucrose gradients and fractions probed for zeta-tubulin, the TRiC/CCT component CCT2, and gamma-tubulin. Molecular weights in kilodaltons (left), and increasing sucrose concentration, left to right, (above) are indicated. Dividing line in E) indicates joint between two membranes, probed and developed identically. See also Table S1 and Figure S1.