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. Author manuscript; available in PMC: 2016 Aug 17.
Published in final edited form as: Curr Biol. 2015 Jul 30;25(16):2177–2183. doi: 10.1016/j.cub.2015.06.063

Figure 2. Zeta-tubulin localizes to the basal foot in multiciliated cells.

Figure 2

A) Dissociated Xenopus multiciliated oviduct cells stained for zeta-tubulin (green), acetylated tubulin (red), and DAPI (blue). Scale bar, 5 μm. B) Transmission EM of oviduct tissue stained with zeta-tubulin antibody and 10 nm gold-conjugated secondary antibody. Arrowhead indicates basal body, and arrow indicates labeling of basal foot. Scale bars, 100 nm. C) Confocal image of live tadpole epidermal multiciliated cell expressing GFP-zeta-tubulin (green), CLAMP-RFP (red), and centrin-BFP (blue). Arrow shows direction of ciliary beating. Scale bar, 5 μm. D) Confocal image of live tadpole epidermal multiciliated cell expressing EMTB-3XGFP (green), mCherry-zeta-tubulin (red), and centrin-BFP (blue). Arrow shows direction of ciliary beating. Scale bar, 5 μm. See also Figures S2 and S3.