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CYP701A8 catalytic activity. A, GC-MS selected ion (m/z = 272 + 288) chromatogram of extract from E. coli engineered for production of syn-pimaradiene with co-expression of CYP99A2 and AtCPR1 (peak 1, syn-pimaradiene; peak 2: syn-pimaradien-3β-ol). B, Mass spectrum for syn-pimaradien-3β-ol. C, Reaction catalyzed by CYP701A8 with syn-pimaradiene.
