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. Author manuscript; available in PMC: 2016 Aug 20.
Published in final edited form as: Mol Cell. 2015 Jul 23;59(4):639–650. doi: 10.1016/j.molcel.2015.06.027

Figure 5. The timing of stress response decline and loss of stress resistance is controlled by jmjd-3.1.

Figure 5

(A) hsp-70 (C12C8.1) and (B) hsp-16.11 expression in wild type (WT), jmjd-3.1 null and jmjd-3.1 over-expressing (o/e) worms exposed to control (c) or HS (33°C, 30 min) conditions. (C) Survival of day 1/day 2 WT (blue/orange), jmjd-3.1 null (red/purple) and jmjd-3.1 o/e (green/black) worms at 35°C. Day 2 WT vs day 2 jmjd-3.1 oe, p = 0.0022; day 1 WT vs day 1 jmjd-3.1 null, p = 0.0096. (D) Thermorecovery of WT, jmjd-3.1 null and jmjd-3.1 o/e animals. (E) hsp-4, (F) gcs-1 and (G) hsp-6 expression in WT, jmjd-3.1 null and jmjd-3.1 o/e worms exposed to control (c) conditions, 33°C heat shock (HS) for 30 min or 200 mM paraquat (para) for 2 hours. (H) Lifespan analysis at 20°C. WT (blue line), jmjd-3.1 null (red line) and jmjd-3.1 o/e (green line). WT vs jmjd-3.1 o/e, p = 0.0006. See table S1 and table S2 for all statistical comparisons. Values plotted are the mean of at least 4 independent experiments and bars represent SEM. Statistical significance was calculated by two-way ANOVA with Bonferroni post analysis comparison except in panel H where log-rank test was used. * p < 0.05, ** p < 0.01, *** p < 0.001. See also figure S5.