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. Author manuscript; available in PMC: 2016 Aug 20.
Published in final edited form as: Mol Cell. 2015 Jul 23;59(4):639–650. doi: 10.1016/j.molcel.2015.06.027

Figure 7. glp-1 mutants maintain the HSR, enhance stress resistance and increase lifespan through jmjd-3.1.

Figure 7

(A) ChIP-qPCR of H3K27me3 at stress gene loci in day 2 adult glp-1(e2141ts) and glp-1(e2141ts);jmjd-3.1(gk384) animals. (B) HS gene expression in glp-1 and glp-1;jmjd-3.1 double mutant animals exposed to control or heat shock conditions at day 2 of adulthood. (C) Thermorecovery (at day 2 of adulthood), (D) thermotolerance (at day 2 of adulthood) and (E) lifespan analysis of wild type (N2) (blue line), glp-1 (red line) and glp-1;jmjd-3.1 (green line) worms raised at 25°C. For (D): glp-1 vs glp-1;jmjd-3.1, p = 0.0013; N2 vs jmjd-3.1(gk384), p = 0.081. For (E): Mean lifespan in days were N2, 12.67 +/− 0.35; glp-1, 15.61 +/− 0.46 and glp-1;jmjd-3.1, 14.2 +/− 0.28; glp-1 vs glp-1;jmjd-3.1(gk384), p = 0.0001. For all statistical comparisons see Tables S1 and S2. Values plotted are the mean of at least 3 independent experiments and error bars represent SEM. Statistical significance was calculated using Student's t-test (A and B), one-way ANOVA (C), two-way ANOVA (D) or log-rank test (E). * p < 0.05, ** p < 0.01, *** p < 0.001, ns p > 0.05. (F) Proposed model for programmed repression of the HSR. Thickness of blue arrows represents relative levels of transcription and intensity of circles represents relative protein levels. Cylinder (nucleosome) proximity represents chromatin accessibility. HSE = heat shock element, sHSP = small heat shock protein. See also figure S7.