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. Author manuscript; available in PMC: 2016 Aug 21.

Published in final edited form as: ACS Chem Biol. 2015 Jun 12;10(8):1791–1796. doi: 10.1021/acschembio.5b00090

Table 2. Kinetics parameters for hydrolysis of carbapenems by OXA-51 and the OXA-51 Trp222Met mutant.

		Antibiotic

Enzyme	Parameter	Imipenem	Meropenem	Ertapenem	Doripenem
OXA-51	k_cat (s^-1)	0.41 ± 0.01	0.113 ± 0.001	0.044 ± 0.001	0.052 ± 0.001
	K_m (μM)	89 ± 7	7.1 ± 0.4	6.0 ± 0.6	5.5 ± 0.6
	k_cat/K_m (s^-1M^-1)	(4.6 ± 0.4) × 10³	(1.6 ± 0.1) × 10⁴	(7.3 ± 0.8) × 10³	(9.5 ± 1.0) × 10³

OXA-51M^a	k_cat (s^-1)	0.28 ± 0.01	0.089 ± 0.001	0.039 ± 0.001	0.053 ± 0.001
	K_m (μM)	4.7 ± 0.3	0.67 ± 0.08	0.51 ± 0.07	0.52 ± 0.09
	k_cat/K_m (s^-1M^-1)	(6.0 ± 0.4) × 10⁴	(1.3 ± 0.2) × 10⁵	(7.7 ± 1.1) × 10⁴	(1.0 ± 0.2) × 10⁵

^a

OXA-51M denotes the OXA51 Trp222Met mutant enzyme.