Table 4. Validation of gene transcription profiles by real-time PCR.

Expression pattern	Gene	Ratio by microarray analysis				Ratio by RT-PCR
		0.5×MIC CYA	MBC CYA	MIC OLA	MBC OLA	0.5×MIC CYA	MBC CYA	MIC OLA	MBC OLA
Conserved	rrn	0.96	0.96	0.96	0.92	1	1	1	1
Up-regulated	recA	7.46	11.42	9.70	13.88	5.97	12.41	8.60	19.05
	lexA	4.07	4.54	4.30	4.68	2.62	2.53	3.18	2.29
	recN	9.95	21.52	15.91	22.24	5.85	9.02	6.03	8.45
	sbmC	2.24	2.74	4.27	2.91	2.54	2.70	2.38	2.17
Down-regulated	rbsC	0.88	0.41	0.72	0.35	1.22	0.49	0.85	0.49
	treB	0.94	0.36	0.65	0.35	1.16	0.40	0.83	0.32
	fliM	0.89	0.45	0.53	0.48	1.29	0.46	0.79	0.49

0.5×MIC, MIC, and MBC indicate drug concentrations.