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. 2015 Aug 21;11(8):e1005381. doi: 10.1371/journal.pgen.1005381

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© 2015 Deng et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — Fluorescence recovery of Actin::GFP after photobleaching. Newly formed Actin::GFP foci in stage 14 embryos were photobleached (arrows) to approximately 30% of the original intensity. The recovery rate was recorded every 3s after photobleaching for a total of 165 sec (endpoint). A. Stills from time-lapse showing Actin::GFP recovery at an actin focus after photobleaching in wild-type and DMef2-Gal4> UAS-diaFH1FH2 embryos. Scale bar: 2.5μM B. Comparison of representative recovery kinetics of Actin::GFP foci in control (green) and DMef2-Gal4> UAS-diaFH1FH2 (red) myoblasts. C. Half time of fluorescent recovery in control (n = 8) and DMef2-Gal4>UAS-diaFH1FH2 embryos (n = 10). The half time of fluorescence recovery in DMef2-Gal4> UAS-diaFH1FH2 embryos (t_1/2 = 17.7±6.7s) is significantly lower than control (t_1/2 = 53.3±6.7s). D. Percentage of final recovery in control and DMef2-Gal4>UAS-diaFH1FH2 embryos. Final recovery in DMef2-Gal4> UAS-diaFH1FH2 embryos (77.9±10.6%) is similar to wild-type embryos (78.6±10.5%) (p>0.1).