Table 2. Comparison of the direct (ITS2-HRM) and indirect (culture/MALDI-TOF) method, expressed as percentage of samples relative to the total number (347) of samples tested.
| Indirect | Total number | ||||
|---|---|---|---|---|---|
| Positive | Negative | ||||
| Direct | Positive | 31.1% (TP) | 12.7 a % (FP) | 152 | 71.1% (PPV) |
| Negative | 2.9 b % (FN) | 53.3 c % (TN) | 195 | 94.9% (NPV) | |
| Total number | 118 | 229 | 347 | ||
| 91.5% (Sensitivity) | 80.8% (Specificity) | ||||
Legend: TP: True positive; FP: False-positive; FN: False-negative; TN: True negative; PPV: positive predictive value, TP/(TP+FP); NPV: negative predictive value, TN/(TN+FN); Sensitivity: TP/(TP+FN); Specificity: TN/(TN+FP)
a No species or less species than the number of species detected with the direct method. This category also contains one sample that was positive only for Achromobacter xylosoxidans with the indirect method, while the direct method showed the presence of Candida albicans.
b No species or less species than the number of species detected with the indirect method
c Two of these samples were positive for Achromobacter xylosoxidans with the indirect method, but are counted as negative for Fungi.