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Nuclear chromatin state was assessed with Hoechst 33342 staining after exposure the cells to 1 μg/ml of the compound PS 1 (A) and PS 2 (B) for 24 h (the concentration value related to the IC50 values of the saponins). The cells treated with the saponins show condensed chromatin relative to the control cells incubated with 0.1% DMSO (C). Arrows represent apoptotic cells.
