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. 2015 Aug 21;10(8):e0136080. doi: 10.1371/journal.pone.0136080

Fig 1. Extracellular ATP upregulates the expression of MRP2 in enterocyte-like Caco-2 cells.

Fig 1

Human intestinal adenocarcinoma Caco-2 cells were stimulated with 100 μM ATP for 3 or 6 h, or with control vehicle only (Ctrl). (A) Stimulation with ATP significantly increased ABCC2 gene expression (encodes for MRP2) by 1.5 to 2-fold compared to non-stimulated Ctrl as determined by qRT-PCR analysis. Results are presented as the mean ± SEM of five individual experiments performed in duplicate. Statistical significance was determined by one-way ANOVA with Dunnett's multiple comparison post-test. * p < 0.05, ** p < 0.01 as compared with Ctrl. (B) Typical Western blot result is showing enhanced MRP2 expression in ATP-stimulated cells. (C) Densitometric analysis revealed that 100 μM ATP induced MRP2 protein expression by more than 1.5-fold after 6 and 18 h of stimulation compared with control (Ctrl). Results are presented as the mean ± SEM of five separate experiments. Statistical significance was determined by one-way ANOVA with Dunnett's multiple comparison post-test. * p < 0.05 as compared with Ctrl.