Fig 7. 224C-F2 is non-toxic to human keratinocytes and murine skin.
(A) Immortalized human keratinocytes (HaCaT cells) were treated with up to 512 μg mL-1 of extract fractions (24 hrs). The LD50 for 224C-F2 could not be determined at this test range, indicating that it is well above the active dose for quorum quenching activity (IC50 = 1.56–25 μg mL-1, depending on strain). (B) Uninfected mice received an intradermal injection of 5 or 50 μg 224C-F2. No gross alterations in skin appearance were observed.