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. 2015 Aug 30;15(8):e29282. doi: 10.5812/hepatmon.29282

Figure 5. Effect of HCV dsRNA-Activated Macrophages SN on the Expression of ISG15, ISG56, MxA, OAS-1, OAS-2 and Viperin in HCV JFH-1-Infected Huh7 Cells.

Figure 5.

HCV JFH-1-infected Huh7 cells (day 3 postinfection) were cultured in the presence or absence of SN from NS-3’NTR dsRNA-stimulated macrophages culture at indicated concentrations for 24 hours. Total cellular RNA extracted from Huh7 cells was subjected to real-time RT-PCR for ISG15, ISG56, MxA, OAS-1, OAS-2 and Viperin mRNA quantification. Data was expressed as RNA levels relative (fold) to control (without SN treatment, which is defined as 1). The results reported as mean ± SD of three independent experiments (* P < 0.05, ** P < 0.01).