Table 1. Primers for Real-Time RT-PCR.
Primer | Sequences (5’ - 3’) |
---|---|
GAPDH | |
Forward | GGTGGTCTCCTCTGACTTCAACA |
Reverse | GTTGCTGTAGCCAAATTCGTTGT |
IFN-α | |
Forward | TTTCTCCTGCCTGAAGGACAG |
Reverse | GCTCATGATTTCTGCTCTGACA |
IFN-β | |
Forward | GCCGCATTGACCATCTATGAGA |
Reverse | GAGATCTTCAGTTTCGGAGGTAAC |
TLR3 | |
Forward | AGCCACCTGAAGTTGACTCAGG |
Reverse | CAGTCAAATTCGTGCAGAAGGC |
IRF-7 | |
Forward | TGGTCCTGGTGAAGCTGGAA |
Reverse | GATGTCGTCATAGAGGCTGTTGG |
ISG15 | |
Forward | GGCTGGGAGCTGACGGTGAAG |
Reverse | GCTCCGCCCGCCAGGCTCTGT |
ISG56 | |
Forward | TTCGGAGAAAGGCATTAGA |
Reverse | TCCAGGGCTTCATTCATAT |
OAS-1 | |
Forward | AGAAGGCAGCTCACGAAACC |
Reverse | CCACCACCCAAGTTTCCTGTA |
OAS-2 | |
Forward | CAGTCCTGGTGAGTTTGCAGT |
Reverse | ACAGCGAGGGTAAATCCTTGA |
MxA | |
Forward | GCCGGCTGTGGATATGCTA |
Reverse | TTTATCGAAACATCTGTGAAAGCAA |
Viperin | |
Forward | TGGGTGCTTACACCTGCTG |
Reverse | TGAAGTGATAGTTGACGCTGGT |
HCV | |
Forward | RAYCACTCCCCTGTGAGGAAC |
Reverse | TGRTGCACGGTCTACGAGACCTC |