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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Dev Biol. 2015 Jul 2;405(1):108–122. doi: 10.1016/j.ydbio.2015.07.001

Figure 5.

Figure 5

Cell proliferation is decreased in MTX treated embryos. A) Schematic of the experimental design. B) Immunohistochemistry of pH3 in representative transverse sections of the face from embryos treated with DMSO (i), or MTX (ii,iii). Images are all at the same magnification, scale bar =100 μm. C) FACS analysis of the cell cycle. i) Representative cell cycle profiles. ii) The average percentages of cells in each fraction from 4 experiments. Statistical significance was determined using Mann Whitney U test and designated by asterisks (p values ≤ 0.028).