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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: J Immunol. 2015 Jul 29;195(5):1939–1943. doi: 10.4049/jimmunol.1500969

Figure 3. cGAS-dependent autoantibodies in Trex1-deficient mice.

Figure 3

(A) Autoantibodies against heart antigens evaluated by blotting neat and 1:5 diluted heart extracts from Rag2−/−Trex1−/− mice with sera from mice of the indicated genotype. Immunoblots were prepared with sera from different mice harvested at the age indicated, rather than with sera harvested repeatedly from a single mouse. Data are representative of two independent experiments. (B) Antinuclear antibodies (ANA) in sera from littermate cGAS+/+Trex1−/−, cGAS+/−Trex1−/−, and cGAS−/−Trex1−/− mice (left panels) analyzed by immunofluorescence staining of HEp-2 cell-coated slides. ANA in serum from cGAS−/−Trex1+/− mice was analyzed as a control (right panel). Data are representative of three independent experiments with a total of at least three mice of each genotype.