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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: J Immunol. 2015 Jul 29;195(5):2294–2302. doi: 10.4049/jimmunol.1500500

FIGURE 3.

FIGURE 3

CD36−/− mice have increased local neutrophil accumulation compared to wild-type mice following LAC supernatant injection. Flow cytometry analysis of single cells suspensions from injection site tissue on day 1 post-LAC intoxication of C57BL6 and CD36−/− mice. (A,B) Presence of Ly6G+ cells, (C) total cell count and (D) F4/80+ cells at the site of intoxication. N=6 mice per group from two independent experiments. (E) il-1b and nlrp3 transcription and (F) IL-1β expression by bone marrow derived neutrophils in response to 1 h incubation with LAC sterile supernatant or media control. N=6 from two independent experiments. (G) Day 1 dermonecrosis, (H) along with MPO and local IL-1β levels, from supernatant intoxicated CD36−/− mice following treatment with anti-Ly6G or isotype control. Values are expressed as percent of isotype control. N=12–15 mice/group from four independent experiments. Data are mean ± SEM. ns, not significant; *, p<0.05; **, p<0.01.