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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: J Immunol. 2015 Jul 22;195(5):2461–2471. doi: 10.4049/jimmunol.1500105

Figure 3. Clinical Isolates of ST Exhibit IFN-β Dependent Suppression of Inflammatory Responses, and Co-operativity with IFN-β is Independent of Cell Death.

Figure 3

(A) WT C57BL6/J peritoneal macrophages were pre-treated for 60 minutes with media alone or 100 u/ml IFN-β and subsequently infected with either ST strain I77 or strain D65 for 6 h. RNA was harvested and expression of CXCL1 and CXCL2 mRNA analyzed by qRT PCR. (B) WT C57BL6/J peritoneal macrophages were pre-treated for 60 minutes with media alone or 100u/ml IFN-β. Additionally half of the cells were pretreated with the cell death inhibitor Necrostatin-1. Macrophages were infected with SL1344 at an moi of 4 for 6 or 24 hours. At each time point samples of cell supernatants were taken and the levels of LDH in the supernatant quantified by colorimetric assay. (C) WT C57BL6/J or caspase-11−/− peritoneal macrophages were pre-treated for 60 minutes with media alone or 100u/ml IFN-β and subsequently infected with either ST strain SL1344 for 6 hours. RNA was harvested and expression of CXCL1 mRNA analyzed by qRT PCR.. Data is representative of three independent experiments. Data are shown as mean ± SEM.