Fig. 2.

Amitriptyline and desipramine induced mitochondrial fragmentation without AIF or cytochrome c release. SH-SY5Y cells were treated with amitriptyline (Ami, 60 μM) or desipramine (Desi, 50 μM). At 24 h after the incubation, the cells were processed for immunostaining using anti-AIF (A) or anti-cytochrome c [Cyto. c, (A, B)] antibodies. As a positive control for the cytochrome c release, SH-SY5Y cells were incubated with 50 μM etoposide [+CTL, (B)] for 12 h. Note that the localization of cytochrome c was confined to the fragmented mitochondria in the amitriptyline-treated cells whereas the cytochrome c was released in etoposide-treated cells in (B). The cells were counterstained with DAPI to visualize nuclei. Scale bar = 10 μm.