Fig. 5.

Subchronically injected amitriptyline induced TH-positive neuronal loss in the SNpc. To examine the in vivo effect of amitriptyline, mice were intraperitoneally injected with amitriptyline (20 mg/kg body weight) twice a week for 4 weeks. Brain tissue sections were prepared from the midbrain area of control vehicle- or amitriptyline-injected mice and immunostained using anti-TH antibodies. Representative immunohistochemistry photographs are shown in (A). (B) The number of TH-positive cells was counted from each SNpc area. At least ten sections from each mouse were counted (n = 5, ^*p < 0.5 vs. vehicle injected).