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Effect of luteolin on different lengths of time on Ang II-induced activation of Akt. Following pretreatment with 50 μM luteolin for 12 h, VSMCs were stimulated with Ang II (1 μM) for the indicated times, and luteolin significantly attenuates Akt phosphorylation induced by Ang II. The cells were lysed and analyzed with antibodies against p-Akt (308), p-Akt (473), and Akt. # P < 0.05 compared with the control group; ∗ P < 0.05 compared with the Ang II group.
