FIGURE 5.

Cav1.3 α1 transcripts containing exons 43_S and 43_L in mouse IHCs and OHCs, at P6 and P22 using nested PCR. Fragments containing 43S (403 bp) or 43L (557 bp) were amplified using nested PCR (see Materials and Methods) with primers specific for exon 42 (forward) and 45 (reverse) of mouse Cav1.3. S1–S14 represent samples from independent preparations. For each cell type and developmental stage at least three independent experiments were performed. Whole brain (WB) and heart (WH) served as positive controls, H₂O (no template) as negative control. Specificity of PCR products was confirmed by sequencing. When two independent PCR reactions with three different RNA samples of each cell type were performed, the number of successful detections for each transcript was as follows: detection of 43L: 6 (out of six experiments) in IHC and OHC preparations of all developmental stages; detection of 43S: 4 (6) in IHC P06 and IHC P22, 6 (6) in OHC P06 and 5 (6) in OHC P22. Bp, basepair markers.