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. 2015 Aug 24;9:309. doi: 10.3389/fncel.2015.00309

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Copyright © 2015 Scharinger, Eckrich, Vandael, Schönig, Koschak, Hecker, Kaur, Lee, Sah, Bartsch, Benedetti, Lieb, Schick, Singewald, Sinnegger-Brauns, Carbone, Engel and Striessnig.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ca²⁺ and Ba²⁺ currents in adult IHCs (P18–21) of Cav1.3DCRD^HA/HA and WT littermates. (A) Selected I_Ca traces (10 mM Ca²⁺) of a WT (black) and a Cav1.3DCRD^HA/HA (HA, gray) mouse IHC after depolarization from a holding potential of -86 mV for 8 ms to the indicated voltages. (B) Corresponding averaged I–V curves from 10 WT (black, mean + SD) and 12 Cav1.3DCRD^HA/HA IHCs (gray, mean – SD) taken between 7 and 8 ms after start of the depolarizing pulse. (C) Representative traces of I_Ca inactivation of WT (black) and a Cav1.3DCRD^HA/HA (gray) IHC during a 300-ms depolarizing step to V_max. The arrow indicates the early phase with faster inactivation absent in Cav1.3DCRD^HA/HA and in Ba²⁺ recordings. (D) Parameters of I_Ca inactivation of 8 WT and 8 Cav1.3DCRD^HA/HA IHCs were obtained by fitting peak current traces from 300 – ms test pulses with a double-exponential function. Individual data points and means (lines) are shown. The fast (τ_fast) inactivation time constant was significantly increased (p < 0.01) and showed a higher variance between individual IHCs in Cav1.3DCRD^HA/HA IHCs. (E) The slow (τ_slow) inactivation time constant was not different between genotypes. (F) Averaged I–V curves of I_Ba with 10 mM Ba²⁺ as charge carrier from 9 WT (black, mean + SD) and 8 Cav1.3DCRD^HA/HA (gray, mean – SD) IHCs between 7 and 8 ms after start of the depolarizing pulse. (G) Representative traces of I_Ba inactivation of a WT (black) and a Cav1.3DCRD^HA/HA (gray) IHC during a 300 ms depolarizing step to V_max.