FIGURE 3.

HRV2 and HRV16 infection lead to mislocalization of nuclear proteins. Ohio-HeLa cells grown on coverslips were infected without (mock) or with HRV2 or HRV16 as for Figure 1; cells were fixed at the indicated times and permeabilized, and then probed with (A) anti-dsRNA and anti-SC35 antibodies, (B) anti-dsRNA and anti-hnRNP-C1/C2 antibodies, or (C) anti-dsRNA and anti-Sam68 antibodies, followed by CF488 and Alexa-568 conjugated secondary antibodies. Coverslips were mounted in ProlongGold mounting media with DAPI. Fluorescence was imaged by CLSM (see Materials and Methods). White arrows indicate infected cells.