Table 2.
Lifetimes obtained from the sequential analysis of the fluorescence decays of the two PSI–LHCI particles measured upon 400 nm and 475 nm excitation with their relative amplitude (i.e. , see Materials and methods) and average decay time τav CS (the ns component is not considered in the calculation, see details and Eq. (1) in Materials and methods). The contributions were set to 0% in case the area under the DAS was negative (see Materials and methods). Some lifetimes were fixed (f) or linked (l).
Small PSI–LHCI | Full PSI–LHCI | |||
---|---|---|---|---|
400 nm | 475 nm | 400 nm | 475 nm | |
τ1 (ps) | 1.0 (f) | |||
Relative amplitude | 0% | |||
τ2 (ps) | 17.6 | 17.3 | 21.4 | 18.2 |
Relative amplitude | 38.5% | 20.4% | 37.7% | 12.3% |
τ3 (ps) | 54.9 | 47.9 | 68.2 | 56.8 |
Relative amplitude | 60.0% | 77.1% | 57.5% | 75.7% |
τ4 (ns) | 1.4 (l) | 0.9 | ||
Relative amplitude | 1.5% | 2.0% | 4.8% | 6.4% |
Average decay time τav CS (ps) | 40.3 | 41.5 | 49.7 | 51.4 |