Figure 4. Pervasive genome-wide chromatin effects of Hairy.

(A) All reduced (top) and increased (bottom) chromatin marks in the genome for H4Ac, H3K27Ac, H3K4me1, H3K4me3, H3K36me3, H3K9me3 and H3 shown as heatmaps for 5 kb windows from the center of significantly affected regions before (−) and after (+) Hairy induction. The number of affected regions indicated below each mark. (B) Affected chromatin regions associated with Hairy-bound genes show preferential enrichments for H4Ac, H3K27Ac, and H3K4me1. All affected regions were assigned to closest genes, and those in the vicinity of Hairy-bound genes are shown. (C) Subset of modified regions from (B) that were linked to genes transcriptionally regulated by Hairy. Significance of enrichment for chromatin modifications shown in Figure 4—figure supplement 1A,B.

DOI: http://dx.doi.org/10.7554/eLife.06394.010

Figure 4—figure supplement 1. Significance of individual histone modifications associated with Hairy bound genes and transcriptionally regulated genes.

(A) Strongest link between loss of H4Ac, gain of H3K4me1, and presence of Hairy on genes. (B) Transcriptionally repressed genes associated with loss of H4Ac, H3K27Ac, and gain or loss of H3K4me1.

DOI: http://dx.doi.org/10.7554/eLife.06394.011

Figure 4—figure supplement 2. Strong correlation between the presence of Hairy binding and chromatin alterations on specific chromatin blocks.

Reduced H4Ac, H3K27Ac, and H3K4me1 significantly associated with Hairy binding. Hairy bound regions overlapped with chromatin blocks by at least 1 bp. y-axis indicates p-value (log_ln).

DOI: http://dx.doi.org/10.7554/eLife.06394.012