Skip to main content

View full-text article in PMC

. 2015 Aug 25;4:e08709. doi: 10.7554/eLife.08709

Table 2.

Intact masses for proteins expressed and purified in this study

DOI: http://dx.doi.org/10.7554/eLife.08709.010

Protein	Vector	Molecular Mass (Da)
Protein	Vector	Calculated	Observed	Δ
Pikp-HMA	pOPINS3C^*	7805.23	7804.97	−0.26
AVR-PikD	pOPINS3C^*	10,835.31	10,832.95	−2.36^§
AVR-PikD	pOPINA^†	10,812.33	10,809.99	−2.34
AVR-PikD	pOPINE^‡	11,786.33	11,784.16	−2.17
AVR-PikE	pOPINS3C^*	10,812.27	10,809.91	−2.36
AVR-PikE	pOPINE^‡	11,763.29	11,760.96	−2.33
AVR-PikA	pOPINS3C^*	10,844.27	10,841.80	−2.47
AVR-PikA	pOPINE^‡	11,795.29	11,793.01	−2.28
AVR-PikC	pOPINS3C^*	10,856.28	10,853.72	−2.56
AVR-PikC	pOPINE^‡	11,807.30	11,804.97	−2.33
AVR-PikD^His46Glu	pOPINE^‡	11,778.30	11,776.07	−2.23
AVR-PikD^Ile49Glu	pOPINE^‡	11,802.28	11,800.04	−2.24
AVR-PikD^Arg64Ala	pOPINE^‡	11,701.22	11,698.94	−2.28
AVR-PikD^Asp66Arg	pOPINE^‡	11,827.43	11,825.31	−2.12
AVR-PikD^Ala67Asp	pOPINE^‡	11,830.34	11,828.20	−2.14
AVR-PikD^{Pro47Ala, Gly48Asp}	pOPINE^‡	11,818.32	11,816.20	−2.12

^*

Non-native residues remaining after 3C cleavage: N-terminal Gly–Pro.

^†

Non-native residues remaining: N-terminal Met.

^‡

Non-native residues remaining after 3C cleavage: N-terminal Gly–Pro; C-terminal Lys-His-His-His-His-His-His.

^§

The measured mass of each AVR-Pik protein should be 2.0156 Da (2 × 1.0078) less than its calculated mass due to formation of the di-sulphide bond.