Figure 5. NF-κB and STAT3, but not MAPKs, are dysregulated by IFNs in STAT1-null macrophages.

Macrophages were incubated with Pam3CSK4 (100 ng/ml) for the indicated times in the absence (a,d) or the presence of 10 ng/ml IFN-γ (b,e) or 10 U/ml IFN-β (c,f). Cells were then lysed and the lysates were immunoblotted with antibodies specific for the indicated proteins. The assays were run under the same experimental conditions and the blots were cropped from original full-sized images (attached as supplemental Fig. S8). Data are representative of at least three independent experiments.