Skip to main content
. 2015 Aug 24;5:13414. doi: 10.1038/srep13414

Figure 6. Combined STAT3 deficiency reverses defective TNF-α production and NF-κB signaling.

Figure 6

(a) STAT1-null BMMs were transfected with control siRNA or siRNA specific for STAT3. Cells were then incubated with Pam3CSK4 (100 ng/ml) for 8 h with or without IFN-γ (10 ng/ml). The production of TNF-α in the supernatant was determined by ELISA. (b) STAT1-null BMMs that were transfected with control siRNA or STAT3 siRNA were stimulated with Pam3CSK4 (100 ng/ml) for 11 h with or without IFN-γ (10 ng/ml). Cell lysates were then immunoblotted with antibodies specific for the indicated proteins. The assays were run under the same experimental conditions and the blots were cropped from original full-sized images (attached as supplemental Fig. S8). Data represent the means ± SD of three independent experiments. *P < .05 and **P < .01.