. 2015 Aug 24;5:13434. doi: 10.1038/srep13434

Table 2. Summary information of the ten proteins tested for immunoglobulin binding.

Protein Name	Ig-bindingCapacity	S. j Protein DatabaseID	NCBI ProteinDatabase ID	Features	Gene CloningPrimers	Expression Vectorand Competentcells	Primers of qRT-PCR
Similar to immunophilin	IgG, IgM	gnl\|lsbi\|CPRT0000001567	AAW27121.1	Surface membrane protein, Immunophilin, Immunogenic protein	F:GGATCCGATGAACAAGGACGCAGT	pET-22b Rosetta(DE3)	F:GCATCTGCTGTAAAACAGGTTC
					R:CTCGAGCAGGGCAGACTTTTCATCATT		R:TCATTCACTACAACCCCATCCT
Sj20.8	IgG, IgM	gnl\|lsbi\|CPRT0000004432	AAP06272.1	EFh superfamily, Tegument antigen,Dynein_light superfamily,Ca²⁺ binding site	F:GGATCCGATGGAACCATTTGTTCAAGT	pET-22b BL21(DE3)	F:TGGCATGTTGTCATAGTTAGAGG
					R:CTCGAGTTCACCGGTCAATTCTTCGT		R: ATTCTTCGTCGGGTGTTCTCC
Sj31	IgG	gnl\|lsbi\|CPRT0000007256	CAX71564.1	Cathepsin,Immunogenic protein	F:GGATCCGATGCTGAACATCGCGTTTTGT	pET-22b TransB(DE3)	F: GGATTACTGGGTATTGCGTGG
					R:CTCGAGCGATTTTATTAGTCCAGCAGCT		R: TTTTGGAAATGGATAGGGTCG
Sj22.6	IgG, IgM	gnl\|lsbi\|CPRT0000007640	AAB52407.1	EFh superfamily,Membrane-associated antigen	F:GGATCCGATGGCAACTACTGAGTACAGATT	pET-22b BL21(DE3)	F: AGTTTTGTCGTGGATTCGGTC
					R:CTCGAGCGAAGACGGTGTTCGCCACAAT		R: CTTTGCCTTCCTTGTCTCTCTT
C1qBP	IgG	gnl\|lsbi\|CPRT0000007943	AAW26757.1	Complement binding protein	F:GGATCCATGTCTCGATTTGTTGCTCGT	pET-28a Rosetta(DE3)	F: GAGGCTCATCCTGATTTGCG
					R:CTCGAGTTCTTTACAGTAATTCTGGAAT		R: GTGTCACCATCCGAGAGTTGTT
Sj-P40	IgG, IgM, IgE	gnl\|lsbi\|CPRT0000008913	AAW24545.1	Major egg antigen	F:GGATCCGATGGATAACTACAGAACAG	pET-22bBL21(DE3)	F: CATCGTCAACTTGGCATCCG
					R:CTCGAGAACTGTGCATAATAAT		R: CCTCCCATTGATACCATCCTTC
Tropomyosin	IgG, IgM	gnl\|lsbi\|CPRT0000009708	CAX72815.1	Immunogenic protein	F:GGATCCGATGGATGGAATTAAAAAG	pET-22b BL21(DE3)	F: GCTCGTCTTGAAGCAGCAGA
					R:CTCGAGATAACCAGTAAGTTCTGCAAAAGT		R: GCTCTTTGTTCAGCAGCCTTTA
SJCHGC06760 protein	IgG, IgM	gnl\|lsbi\|CPRT0000009944	AAW25344.1	Annexin superfamily,Ca²⁺ binding site	F: GGATCCATGGCTAAAGTTTCT	pET-28a Rosetta(DE3)	F: ATCGGATACCAGTGGGGATT
					R: CTCGAGTTCACCAAGTAGAACAC		R: GCATTGGCTACTGCTCTCATT
Annexin A13	IgG, IgM	gnl\|lsbi\|CPRT0000011452	CAX70810.1	Annexin superfamily,Ca²⁺ binding site	F:GGATCCATGGGAAGATCTAAAATTC	pET-28a Rosetta(DE3)	F: GATGCTGGTGAAGCACGTCTA
					R:CTCGAGACTCCATTCAGCACCAATT		R: CAATCGCTTGTATATGCCAAAC
Sjc23-LED	IgG	gnl\|lsbi\|CPRT0000000982	P27591.1	CD63 antigen,Tetraspanin	F:GGATCCGTACAAGGATAAAATCGATG	pET-22b BL21(DE3)	F: ATGACTGGTGCTCTGGAAAATC
					R:CTCGAGGTTGCGTTTTAAGAATGCACTAAAG		R: CCTGAACATAAACTTCTTGCCC