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. 2015 Aug 24;5:13396. doi: 10.1038/srep13396

Figure 4. METH-induced increased enrichment of HDAC2 on IEG promoters in WT mice.

Figure 4

Chromatin immunoprecipitation (ChIP) assay was used to measure HDAC2 abundance on the promoters of (A) Fosb, (B) Fra2, (C) Egr2, (D) Egr3, (E) Nr4a2, and (F) Nr4a3. Increased HDAC2 binding was observed on Fosb, Fra2 and Egr3 promoters at 2 hours after METH, corresponding to a time when the mRNA levels of these genes were reverting towards normal values. The relative amounts of HDAC2-immunoprecipitated DNA were normalized to 10% of input control and expressed as fold-changes in comparison to saline-injected mice. Values represent means ± SEM (N = 7–9 per time point). Key to statistics: *p < 0.05, **p < 0.01, significantly different from saline treated WT mice, ##p < 0.01; ###p < 0.001 in comparison to METH-treated WT mice at the 1-hr time point.