Table 1.
Summary of SAXS experiments
| Data Collection | |
|---|---|
| Beamline | SSRL Beamline BL4-2 |
| Defining slit size (mm) | 0.3(H) × 0.3 (V) |
| Beam energy (keV) | 11 |
| Sample-detector distance (m) | 1.7 |
| Detector | Rayonix MX225-HE |
| Pixel binning | 8 × 8 |
| Pixel size (μm) | 292 |
| Exposure time (s) | 1 |
| Images | images taken for duration of run =∼700 images |
| Type of sample cell | quartz capillary (diameter 1.5 mm) |
| Temperature (K) | 283 |
| Calibrant | AgBe |
| Final q range (Å−1) | 0.01 to 0.5 |
| Data Analysis | |
| Programs | SASTOOL (47), PRIMUS (48) |
| Buffer | 20 mM Tris, 50 mM NaCl, pH 7.5 |
| Injected protein concentrations (mg/mL) | see Table 2 |
| Points used for Guinier analysis | see Table 2 |
| Guinier qRg limits | see Table 2 |
| Guinier Rg (Å) | see Table 2 |
| GNOM q range (Å−1) | see Table 2 |
| Dmax (Å) | see Table 2 |
| Rg (real) (Å) | see Table 2 |
| Rg (reciprocal) (Å) | see Table 2 |
| Modeling | |
| Programs | IMP (rrt_sample, foxs, multi_foxs), Pymol |
| Data plotting | GNUPLOT |