Table 2.
Summary of data analysis of the experimental SAXS profiles
| Samples | Injected Concentrationa (mg/mL) | FPLC Flow Rate (mL/min) | Rg (Real ± SD) (Å) | Guinier Points | q∗Rg | Dmax (Å) | Native Molecular Weight (kDa) | Molecular Weight Estimationb (kDa) | Porod Volumec (Å3) (q-range (Å−1)) |
|---|---|---|---|---|---|---|---|---|---|
| recPrP(89–230) | 3.6 | 0.05 | 21.84 ± 0.88 | 13–44 | 1.081 | 76.5 | 16.2 | 15.4 | 20,548 (0.010–0.255) |
| recPrP(23–230) | 4.1 | 0.08 | 30.69 ± 1.20 | 1–18 | 0.495 | 104.4 | 22.9 | 26.0 | 39,832 (0.017–0.258) |
| Fab-P | 5.3 | 0.05 | 26.86 ± 0.12 | 9–43 | 1.305 | 84.8 | 47.4 | 48.4 | 67,280 (0.010–0.150) |
| Fab-R1 | 4.1 | 0.05 | 26.25 ± 1.10 | 20–45 | 1.301 | 96.8 | 47.5 | 48.8 | 68,292 (0.010–0.142) |
| recPrP(89–230)-Fab-P | 1.0 | 0.05 | 36.20 ± 0.57 | 3–15 | 0.850 | 121.1 | 63.6 | 68.6 | 84,955 (0.010–0.191) |
| 2.6 | |||||||||
| recPrP(23–230)-Fab-P | 1.0 | 0.08 | 38.90 ± 0.60 | 1–15 | 0.986 | 136.1 | 70.3 | 73.8 | 104,183 (0.013–0.217) |
| 3.7 | |||||||||
| recPrP(89–230)-Fab-R1 | 1.0 | 0.05 | 34.40 ± 0.48 | 1–16 | 0.914 | 120.4 | 63.7 | 62.8 | 78,474 (0.013–0.197) |
| 3.7 | |||||||||
| recPrP(23–230)-Fab-R1 | 1.0 | 0.08 | 33.20 ± 0.83 | 1–10 | 0.904 | 113.6 | 70.4 | 62.2 | 79,039 (0.019–0.205) |
| 2.9 |
a
We estimate that the eluted concentration at the beam capillary is ∼67% of the corresponding injected concentration, according to UV spectroscopy (data not shown).
b
Molecular weights were estimated using SAXS MOW (49) with a threshold of Qmax = 0.25 Å−1.