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. 2015 Aug 18;109(4):722–731. doi: 10.1016/j.bpj.2015.06.063

Figure 2.

Figure 2

GlpF incorporation and activity in diC18:1-PC liposomes with increasing diC18:1-PE content. (A) Relative GlpF incorporation. Error bars: SD (N = 6). (B) Representative light-scattering curves of GlpF activity measurements at 0 (black), 40 (light gray), and 60 (gray) mol % diC18:1-PE. Each curve is the average of five measurements. (C) Normalized weighted rate constants of ribitol flux in liposomes of increasing mol % diC18:1-PE. Error bars: SD (N = 6). The SD of GlpF activity in diC18:1-PC liposomes was calculated from the relative weighted rate constants (kw,rel.), and the SD of GlpF activity in the other liposomes was calculated from the normalized weighted rate constants (kw,norm.). The PE concentration in the native E. coli inner membrane is ∼70–75%.