Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Aug 13;16(Suppl 11):S9. doi: 10.1186/1471-2105-16-S11-S9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 Pretorius et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Parameter optimisation for colour deconvolution of a histology image (see case study). (a) The original H&E stained image of a liver section. (b) Deconvolution result of the hematoxylin stain using default values. (c) Deconvolution result of the eosin stain using default values. (d) Deconvolution result of the hematoxylin stain using more optimal values found using our method. (e) Deconvolution result of the eosin stain using more optimal values. Note that the aim is not feature detection but accurate isolation of individual dyes, which have overlapping absorption rates in different sub-cellular structures. Results (d) and (e) reflect the absorption rates of the component stains more accurately than (b) and (c).