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. 2015 Aug 3;112(33):10491–10496. doi: 10.1073/pnas.1512424112

Table S3.

Plasmids

Plasmids Description Source
pRbmA FLAG-tagged full-length RbmA in pFLAG-CTC (16)
pRbmA* FLAG-tagged RbmA* in pFLAG-CTC This study
p[Sec]RbmA FLAG-tagged RbmA without the Sec signal in pFLAG-CTC This study
p[Sec+]RbmA* FLAG-tagged RbmA* with the Sec signal in pFLAG-CTC This study
pRbmA*-RTX RbmA fused to RtxA T1SS in pFLAG-CTC This study
pRbmA-RTX RbmA* fused to RtxA T1SS in pFLAG-CTC This study
pFLAG-CTC Control vector Sigma
pBADLacZ Control vector Invitrogen
pTagA VC0820 in pBAD-TOPO This study
pVCA0148 VCA0148 in pBAD-TOPO This study
pHAP VCA0865 in pBAD-TOPO This study
pPrtV VCA0223 in pBAD-TOPO This study
pVC1650 VC1650 in pBAD-TOPO This study
pVC0157 VC0157 in pBAD-TOPO This study
pVesA VCA0803 in pBAD-TOPO This study
pVesB VCA1200 in pBAD-TOPO This study
pVesC VC1649 in pBAD-TOPO This study
pWM91 oriR6KmobRP4 lacI pTac tnp miniTn10Km B. Wanner, Purdue University,  West Lafayette, IN
pWM91::ΔrbmA pWM91 carrying a fragment of rbmA harboring an unmarked internal deletion (16)
pWM91::rbmA-FLAG pWM91 encoding the C terminus of RbmA fused to a FLAG-tag with flanking DNA for recombination onto the chromosome This study
pWM91::ΔhapA pWM91 carrying a fragment of hapA harboring an unmarked internal deletion This study
pWM91::ΔrbmA pWM91 carrying a fragment of rbmA harboring an unmarked internal deletion This study
pWM91::ΔprtV pWM91 carrying a fragment of prtV harboring an unmarked internal deletion This study
pWM91::ΔVC0157 pWM91 carrying a fragment of VC0157 harboring an unmarked internal deletion This study
pGP704::rbmA-FLAG pGP704 carrying the 3′ end of rbmA encoding a FLAG-tag; Apr (16)