Fig. S4.
Allelic variation at ChFLC affects the coordination between leaf morphology and flowering time in C. hirsuta. The consequences of FLC variation on leaf morphology are more pronounced in C. hirsuta than A. thaliana. (A) The shape factor [perimeter2/(4π*area)] for leaf 8 is shown to compare the effects of distinct FLC alleles on leaf shape in C. hirsuta and A. thaliana. (B) Heteroblastic increase of leaflet number in NIL_Ox plants grown in LD for 0 d (red), 9 d (blue), and 25 d (green) and then transferred to SD (n = 15 per genotype). Leaflet number of all rosette leaves produced before flowering is shown. (C and D) Leaflet number (C) and total rosette leaf number (D) of NILs grown in SD. (E and F) Leaflet number (E) and total rosette leaf number (F) of NILs grown in LD. (G and K) qRT-PCR analysis of the expression levels of ChFT (G), ChSPL3 (H), ChSPL9 (I), ChSPL13 (J), and ChSPL11 (K) along a developmental time series. *P < 0.01 (Student’s t test). Data are represented as mean values with SD.