. 2015 Aug 4;112(33):10539–10544. doi: 10.1073/pnas.1419791112

Table S5.

List of primers used for gene cloning, qRT-PCR analysis, and dideoxy sequencing

Experiment	Gene	Primer_F	Primer_R
Gene cloning	FLC_Col-0	`TTCCTTAGTGTCGGCTGGAT`	`ATGCCGTACATTCACCAAT`
Gene cloning	FLC_Wa	`CACTCGTTCACGAGAAAAGG`	`CACGACGCGTTTTGAAATAA`
qRT	ChGADPH	`TGACCACCGTCCACTCCATCAC`	`GCTCTTCCACCTCTCCAGTCCTTC`
qRT	ChFT	`AGGTCCTCTCCACCAATCTCAACTC`	`TGTAAGAGACCCTCTTCTGGTCAGC`
qRT	ChSOC1	`TCAATCGAGGAGCTGCAACA`	`TCCGAACTTGGGCTACTCTC`
qRT	ChFLC	`AACCCAAACCTCACCTCAGGATCAAATTAGG`	`CGGCTACTTTTGTTCTCAATTCGC`
qRT	ChSPL3	`GAAGGAGAAGAAGATTCTGAGGACACT`	`GTTGGTGAAGACCAGAGATCCGA`
qRT	ChSPL9	`GTGGAATGAACAATGGAAGC`	`ATGAAAAGCTTGTCCCTCCT`
qRT	ChSPL13	`CTTCATCCTCTGTCCCGCAT`	`GTGGAAGAGCAACAGCCTCA`
qRT	ChSPL11	`GTCCAAGTTTCAACTTCCTGGCG`	`AGAATAGAGTAGAGAAAGCGGCTGC`
qRT	ChSPL15	`ATATGGCGAGCATCTTCAGG`	`TGTGGTGCGATTTCTCATGT`
Sequencing	ChFLC_F1/R1	`TAGCGGCAGCATTATCGAATCGG`	`TATCACTCTGGTAGAGATTCC`
Sequencing	ChFLC_F1seq	`AAACGTTATTAGTCATTCATTAGCGGG`
Sequencing	ChFLC_F2/R2	`TAGAGTTCAAAGACAAATGAAAATGG`	`TAAAGTAGAGATCACAATATGTAGC`
Sequencing	ChFLC_F3/R3	`ACTAAATTAATACTCCTAGACTCC`	`AACTAAATCGCTATTACAACTAACC`
Sequencing	ChFLC_F4/R4	`ATCTTTATGATTTGTTTGTTTTAGGTCC`	`TAGTTAAGAAACTAGATACAATACAAGAGG`
Sequencing	ChFLC_F5/R5	`TCTAGAATCAGTTTGGGTTTCGTTTCG`	`ACGTGTACCGCATGACTAATTCCCAGG`
Sequencing	ChFLC_F6^*/R6	`AGTATAAATTTGAGACTAAAAGAGG`	`TTGTCGGCTACTTTTGTTCTCAATTCG`
Sequencing	ChFLC_F7/R7^*	`AACGCTCAGTATCTCCGGCGACTTGAACCC`	`TTTAGCTCATCACGACATAGTTCTTGC`
Sequencing	ChFLC_F8^*/R8	`TTCCTTATATGGGCTTTCATTTATTTCCC`	`TAGATCTCTCGGAAGTACACTGCATTGC`
Sequencing	ChFLC_F9/R9^*	`AAGTTGCATGTCCTTCAAGATCTTTTCG`	`AAGATGTTTATATATACACACATCAACTGC`
Sequencing	ChFLC_F10/R10	`ATCCAAATTATGGTTTCTGATTGATTCC`	`AATTAAGGTCGTGTTGTAAATTTCC`
Sequencing	ChFLC_F11/R11	`AAATGTATGCTACATTGTGCAACTATTGC`	`TTTGCACGAAAATCAAGGATGCACAAACG`
Sequencing	ChFLC_F12^*/R12^*	`ATTTGTTCTTGCCTTATATTGAATCC`	`AAACGACTTCCCCAGTAGTCTTCCACTTTCC`
Sequencing	ChFLC_F13/R13	`TTAACTTTCAGTGGCAGAAAAATCTCTACC`	`TATATTTAAACCCGAAAATCAAACAATCAAGCC`
Sequencing	ChFLC_F14^/R14^	`TAACCACTGGCTCTAATATCACAATTTGTGG`	`AAAGGAGATCTAATATTTACCAAGTATTGC`
Sequencing	ChFLC_F15/R15	`AATTGATAGGTTACAACATGTACTGG`	`ATCTGGAACTCAGGCCTTAAGTAAATTTTGG`
Sequencing	ChFLC_F16/R16	`TTCAGAGTTAGCTAGTAGCTTTGATCC`	`AAGGTCTAGTAGCTCGTGGTGTGAACC`
Sequencing	ChFLC_F17/R17	`ACCTTGACATTGTGGAGATCTAGATCC`	`TACTTTAAACTTGGAGGCAAATATCC`
Sequencing	ChFLC_F18/R18	`TAACTAGAGCCAAGAAGGTAAGTTGATTCC`	`AATGCTAATTAGTGAGTGCTTAGGGTGC`
Sequencing	ChFLC_F19/R19	`TTGGCTAACCAGGTAATTAACGAAAGC`	`TTGTTTTTGCTAAAACCCTGACTTCATCGG`
Sequencing	ChFLC_F20/R20	`TTTTATTGATAAAACTATACATACACACATAC`	`AACCCAGGGAATATTCTGTTCATACAACC`
Sequencing	ChFLC_F21/R21	`ACATGCTCAAGGTGGACAAGGTTACATGC`	`CAGGTGACCGACGTAGGCCCATAGAAACC`
Sequencing	ChFLC_F21seq	`AGTACACACAACGTCTCATTTTTGC`

These primers were used to sequence parts of the first intron of ChFLC in the Gr-1 and Jpa1 strains.