Skip to main content
. 2015 Aug 3;112(33):10413–10418. doi: 10.1073/pnas.1506101112

Table S1.

Yeast strains used in this study

Strain name Genetic background Relevant genotype Source
PT95-19C W303* MATa ADE2 TRP1 FOB1-CFP Present study
PT19-1C W303 MATa HTA1-CFP::NAT Present study
W8164-2B W303 MATα CEN1-16::Gal-Kl-URA3 (32)
PT73-7A W303 MATa CSE4-CFP::HIS3 Present study
PT129-1A W303 MATα ADE2 LYS2 SPC110-CFP::KAN RAD5 Present study
GFP strains BY4741 MATa his3∆1 leu2∆0 met15∆0 ura3∆0 XXX-GFP::HIS3 (20)
T317 BY4741 MATa CDC14-GFP::HIS3 ubr2∆::NAT Present study
T306 BY4741 MATa NDC10-GFP::HIS3 ubr2∆::NAT Present study
T307 BY4741 MATa BIR1-GFP::HIS3 ubr2∆::NAT Present study
T310 BY4741 MATa NNF1-GFP::HIS3 ubr2∆::NAT Present study
T311 BY4741 MATa DAD2-GFP::HIS3 ubr2∆::NAT Present study
T309 BY4741 MATa NSL1-GFP::HIS3 ubr2∆::NAT Present study
T312 BY4741 MATa DAD2-GFP::HIS3 ubr2∆::NAT Present study
T308 BY4741 MATa NUF2-GFP::HIS3 ubr2∆::NAT Present study
PT142-1C W303 MATα ADE2 TRP1 leu2-3,112 his3-11,15 ura3-1 lys2∆ RAD5 ASK1-CFP Present study
PT95-35A W303 MATα ADE2 trp1-1 leu2-3,112 his3-11,15 FOB1-CFP Present study
PT39-30A W303 MATα ADE2 TRP1 leu2-3,112 his3-11,15 ura3-1 lys2∆ RAD5 MTW1-CFP Present study
PT149 W303/BY4741 MATα/MATa FOB1-CFP CDC5-GFP::HIS3 Present study
PT150 W303/BY4741 MATα/MATa FOB1-CFP TOM70-GFP::HIS3 Present study
PT151 W303/BY4741 MATα/MATa FOB1-CFP YPR174C-GFP::HIS3 Present study
PT152 W303/BY4741 MATα/MATa FOB1-CFP NUP1-GFP::HIS3 Present study
PT153 W303/BY4741 MATα/MATa FOB1-CFP CDC14-GFP::HIS3 Present study
PT154 W303/BY4741 MATα/MATa MTW1-CFP CDC14-GFP::HIS3 Present study
PT155 W303/BY4741 MATα/MATa ASK1-CFP CDC14-GFP::HIS3 Present study
PT156 W303/BY4741 MATα/MATa ASK1-CFP CDC5-GFP::HIS3 Present study
PT157 W303/BY4741 MATα/MATa ASK1-CFP TOM70-GFP::HIS3 Present study
PT158 W303/BY4741 MATα/MATa ASK1-CFP YPR174C-GFP::HIS3 Present study
PT159 W303/BY4741 MATα/MATa ASK1-CFP NUP1-GFP::HIS3 Present study
T376 BY4741 MATa NNF1-GFP::HIS3 DSN1-3HA::HYG Present study
T81 BY4741 MATa CSE4-GFP::HIS3 mad3∆::KAN Present study
T82 BY4741 MATa CDC20-GFP::HIS3 mad3∆::KAN Present study
T83 BY4741 MATa NDC10-GFP::HIS3 mad3∆::KAN Present study
T84 BY4741 MATa IPL1-GFP::HIS3 mad3∆::KAN Present study
T85 BY4741 MATa BIR1-GFP::HIS3 mad3∆::KAN Present study
T414 BY4741 MATa NUF2-GFP::HIS3 mad3∆::KAN Present study
T415 BY4741 MATa NNF1-GFP::HIS3 mad3∆::KAN Present study
T416 BY4741 MATa MTW1-GFP::HIS3 mad3∆::KAN Present study
T417 BY4741 MATa CDC14-GFP::HIS3 mad3∆::KAN Present study
T418 BY4741 MATa KRE28-GFP::HIS3 mad3∆::KAN Present study
T419 BY4741 MATa NSL1-GFP::HIS3 mad3∆::KAN Present study
T420 BY4741 MATa CEP3-GFP::HIS3 mad3∆::KAN Present study
T421 BY4741 MATa DAD3-GFP::HIS3 mad3∆::KAN Present study
T422 BY4741 MATa BIK1-GFP::HIS3 mad3∆::KAN Present study
T423 BY4741 MATa BUB3-GFP::HIS3 mad3∆::KAN Present study
T424 BY4741 MATa MIF2-GFP::HIS3 mad3∆::KAN Present study
T425 BY4741 MATa CBF1-GFP::HIS3 mad3∆::KAN Present study
T426 BY4741 MATa SLI15-GFP::HIS3 mad3∆::KAN Present study
T427 BY4741 MATa AME1-GFP::HIS3 mad3∆::KAN Present study
T428 BY4741 MATa MCM21-GFP::HIS3 mad3∆::KAN Present study
T429 BY4741 MATa CTF19-GFP::HIS3 mad3∆::KAN Present study
T430 BY4741 MATa STU2-GFP::HIS3 mad3∆::KAN Present study
T431 BY4741 MATa DSN1-GFP::HIS3 mad3∆::KAN Present study
T432 BY4741 MATa mtw1∆::KAN with plasmid pHT10 [MTW1-GBP LEU2] Present study
T449 W303 MATα CEN1-16::Gal-Kl-URA3 CDC14-GBP-RFP::KAN Present study
PT209-7A W303 MATa ura3::3xURA3-tetOx112 TetR-mRFP(iYGL119W) MTW1-YFP Present study
*

W303 strains are ADE2+ RAD5+ derivatives of W303 (can1-100 his3-11, 15 leu2-3,112 ura3-1), unless otherwise indicated (56, 57).

GFP strains are all based upon BY4741 [his3∆1 leu2∆0 met15∆0 ura3∆0 (20)].