Fig. 2.

CRISPR/Cas9 modeling of CDK2 SNP rs3087335. (A) Domains in the hCDK2 protein and location of rs3087335 (red arrow). (B) CRISPR/Cas9 editing strategy for making the Y15S alteration. The Tyr15 site is conserved in humans and mice. PAM nucleotides are in green. Underscored nucleotides match the CRISPR gRNA. To avoid Cas9 recutting of an already-edited locus, two silent mutations (blue) were included into the ssODN recombination template, only part of which is shown (Table S1). (C) Sanger sequencing chromatograms of control and mutant mice showing correct CRISPR/Cas9 targeting.