Fig. 3.
The CDK2Y15S alteration encoded by rs3087335 causes male infertility. (A) Testes (90 d old) from littermates of indicated genotypes. (B) Testes weights of P90 heterozygous and homozygous mice were 54.6% and 81.5% lower, respectively, than those of WT. (C–E) H&E-stained seminiferous tubule cross-sections of 15-, 25-, and 90-d-old testes. Arrowheads indicate degenerating cells. (F) Sperm counts of 3- and 6-mo-old WT and heterozygous males. (G) Western blot analysis of CDK2 in spleen. Genotypes are indicated, as are approximate molecular weights of the detected species with anti-CDK2 antibody. Spleens were used instead of testes, because differences in cellularity of mutant testes would compromise interpretation of relative protein amounts. ****P < 0.0002; ***P < 0.0001; **P < 0.001. Data are presented as mean ± SD of at least three animals. Ser, Sertoli cells. (Scale bars: 100 μm).