. 2015 Aug 3;112(33):10431–10436. doi: 10.1073/pnas.1506974112

Table S2.

Efficiency of CRISPR/Cas9-mediated targeting

Gene	Concentration (Cas9/sgRNA/ ssODN), ng/μL	Two-cell stage embryos/zygotes surviving injection, n (%)	Recipients, n	Edited animals/ newborns, n (%)	HDR edited mice, n (%)	HDR heterozygotes/ HDR edited mice, n
Cdk2^*	50/50/100	69/86 (80.2)	3	20/27 (74.1)	10/27 (44.4)	2/10
Mlh1	100/50/100	95/135 (70.3)	4	9/24 (37.5)	7/24 (29.1)	4/7
Smc1b	100/50/100	101/150 (67.3)	4	5/14 (35.7)	3/14 (21.4)	2/3
Tex15^†	50/50/100	35/40 (87.5)	2	16/16 (100)	9/16 (56.2)	3/9

The number of edited animals includes those containing both indels and precise (HDR) alterations.

As reported previously (5).

^{^†}

After microinjection, embryos were cultured to the two-cell stage in KSOM supplemented with 50 μΜ SCR7 as reported previously (5).