Fig. 4.

Evaluation of structural elements that distinguish polymerase activities. Polm^−/−Poll^−/− cells were complemented by introducing the indicated human WT or mutant polymerases together with (A) G3′ and GCG3′, (B) GACG3′, or (C) GCG3′ and GCAG3′ substrates. Either 10 ng (Pol μ constructs) or 1 ng (Pol λ constructs) was used for complementation. The percentage of the indicated synthesis-associated products was determined by sequencing, with error bars derived as in Fig. 1.