Fig. S3.

Synthesis at ends that align to generate two nucleotide gaps. The GCAG3′ substrate (described in cartoon) was introduced into MEFs as described in Fig. 1B, but using a panel of MEFs generated independently, derived from mice from different litters. Joining efficiency was determined by qPCR, but the proportion of recovered junctions formed after synthesis of TC was approximated by assessing the fraction of products sensitive to a restriction enzyme specific for this junction (AatII). Error bars reflect the range of means from two triplicate transfections, performed on different days. ND, not detectable (recovery efficiency < 0.01).