Table S1.

Primers and restriction enzymes used to generate substrates

Substrate	Primers	Restriction enzymes
G3′	5′-CAAGTGGACCACATGTCTTAGCTGTATAGTCAGGGAAATC-3′	AhdI
	5′-CCGCCGACGCCATGTCACACCCATCTCAGACTGGCTACCC-3′
G3′-Blunt	5′-TCAGTATCCTGTGACTGCACACCCATCTCA-3′	BciVI/PvuII
	5′-GTGGTCAGCTGGCTTAGCTGTATAGTCAG-3′
GCG3′	5′-TTTTTGCCACGCTGGCTTAGCTGTATAGTCAGGGA-3′	BglI
	5′-CACCTGCCTCGCTGGCACACCCATCTCAGACTGGC-3′
TG3′	5′-TTTTTGCAATGCAATCGCTTAGCTGTATAG-3′	BsrDI
	5′-TCACGCAATGCAATCACACCCATCTCA-3′
GACG3′	5′-CAAGTGGTCCACCGTCATGGCTTAGCTGTATAG-3′	BstXI
	5′-GCCGACCAGCGTCATGGCACACCCATCTCA-3′
5′GCAA	5′-CAAGTGGTCTCCGCAAATCGCTTAGCTGTATAG-3′	BsaI
	5′-GCCGAGGTCTCAGCAAATCACACCCATCTCA-3′
5′GCA	5′-TCATGCTCTTCCGCAATCACAGAAAATCGCTTAGCTG-3′	BspQI
	5′-CAAGGCTCTTCAGCAATCTGGACGAATCACACCCATCT-3′