Fig. 1.
Hydrogenase determinants in P. methylaliphatogenes strain K22T. (A) Phylogenetic tree showing the phylogeny of the P. methylaliphatogenes hydrogenase large subunit sequence compared with those of Group 5 [NiFe]-hydrogenases. The tree was constructed using the neighbor-joining method, bootstrapped with 500 replicates, and rooted using representatives of the Group 1 and Group 2 [NiFe]-hydrogenases. (B) RT-PCR analysis determining the structure of the operon encoding the hydrogenase structural components. The structure was determined by detecting the presence of intergenic PCR products for cDNA samples against positive controls (gDNA) and negative controls (mRNA). Primers were designed to amplify the start, end, and intergenic regions of adjacent genes (loci numbers shown above the lane). (C) To-scale structure of the elucidated operon showing locus numbers and functional annotations of the genes.