Fig. 2.

Hydrogenase expression in P. methylaliphatogenes strain K22^T. (A) Growth of P. methylaliphatogenes in FS1V minimal medium supplemented with 2.5 mM glucose. Cells were harvested for RNA extraction during exponential phase (OD₆₀₀ = 0.1) and stationary phase (t = 120 h). (B) Glucose concentration of the external medium during cell growth. (C) Expression ratios of the genes encoding the hydrogenase small and large subunit during stationary vs. exponential phase as determined by qRT-PCR and normalized to 16S rRNA gene expression. In both cases, error bars represent SDs from three biological replicates.