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. 2015 Aug 3;112(33):10497–10502. doi: 10.1073/pnas.1508385112

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Fig. S1. — Multiple sequence alignments (Clustal) of the genes encoding the large subunits of the Group 5 [NiFe]-hydrogenases in P. methylaliphatogenes (PYK22_03065), Solibacter usitatus (Acid_6556), Mycobacterium smegmatis (MSMEG_2719), Streptomyces avermitilis (SAV_7367), and Ralstonia eutropha (PHG065). The four cysteine residues highlighted in yellow ligate the [NiFe] center that serves as the catalytic center. Sequences in red are predicted to be cleaved by the HupD endopeptidase during hydrogenase maturation.