Table S1.
Genes associated with the hydrogenase-encoding locus of P. methylaliphatogenes strain K22T
| Locus | Gene name | Proposed function |
| Structural operon | ||
| PYK22_03064 | hhyS | Hydrogenase small subunit |
| PYK22_03065 | hhyL | Hydrogenase large subunit |
| PYK22_03066 | hhyE | Putative [2Fe2S] electron transfer protein |
| PYK22_03067 | hhaA | Conserved hypothetical protein |
| PYK22_03068 | hhaB | Conserved hypothetical protein |
| PYK22_03069 | hhaC | Conserved hypothetical protein |
| PYK22_03070 | Unconserved hypothetical protein | |
| PYK22_03071 | hupD | Hydrogenase apoprotein endopeptidase |
| Upstream genes | ||
| PYK22_03060 | Homologous to hhyS, possibly sensory or nonfunctional | |
| PYK22_03061 | PAS domain-containing histidine kinase | |
| PYK22_03062 | LuxR-like response regulator | |
| PYK22_03063 | Putative transmembrane protein | |
| Downstream genes | ||
| PYK22_03072 | Predicted nonconserved 43-residue peptide | |
| PYK22_03073 | YceI-like transmembrane protein | |
| PYK22_03074 | hypA | Ni2+ insertion into hydrogenase large subunit |
| PYK22_03075 | hypB | Ni2+ insertion into hydrogenase large subunit |
| PYK22_03076 | hypC | Transfer of Fe-(CN−)2-(CO) Moiety to Large Subunit |
| PYK22_03077 | Conserved hypothetical protein | |
| PYK22_03078 | hypD | Transfer of Fe-(CN−)2-(CO) Moiety to Large Subunit |
| PYK22_03079 | hypE | Biosynthesis of CN− Ligands |
| PYK22_03080 | gmhA | Phosphoheptose Isomerase |
| PYK22_03081 | Conserved hypothetical protein | |
| PYK22_03082 | hypF | Biosynthesis of CN− Ligands |